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anti-myosin heavy chain type iib  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank anti-myosin heavy chain type iib
    Anti Myosin Heavy Chain Type Iib, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 98/100, based on 844 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myosin+iib/anti-Myosin+heavy+chain+Type+IIB/custom%40bf%2Ef3%4041770536
    Average 98 stars, based on 844 article reviews
    anti-myosin heavy chain type iib - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: A central role for vimentin in regulating repair function during healing of the lens epithelium
    Article Snippet: Cells were incubated with primary antiserum followed by rhodamine-conjugated (Jackson ImmunoResearch Laboratories, West Grove, PA, and Millipore), fluorescein-conjugated (Jackson ImmunoResearch Laboratories), or Alexa Fluor 488–conjugated (Invitrogen-Molecular Probes, Carlsbad, CA) secondary antibodies. .. The following primary antibodies were used for the immunofluorescence studies: vimentin (mAb; Developmental Studies Hybridoma Bank), vimentin (polyclonal) antibody (a generous gift from Paul FitzGerald), paxillin (mAb; BD Transduction Laboratories, Franklin Lakes, NJ), β4 integrin (polyclonal) antibody as described in Sta Iglesia et al. (2000), α6 integrin (mAb; Covance, Princeton, NJ) myosin IIA (polyclonal, Sigma-Aldrich), myosin IIB (mAb; Developmental Studies Hybridoma Bank), αtubulin (mAb; Developmental Studies Hybridoma Bank), and α-tubulin (polyclonal) antibody (Cell Signaling, Danvers, MA). .. Some explants were counterstained with Alexa Fluor 488–conjugated phalloidin, which binds filamentous actin, and TO-PRO 3, a nuclear stain (Invitrogen-Molecular Probes).

    Immunohistochemistry:

    Article Title: Sema3a-Nrp1 signaling mediates fast-twitch myofiber-specificity of Tw2 + cells
    Article Snippet: For immunohistochemistry of fixed frozen sections, the following antibodies were used: Twist2 (Abcam, ab66031, 1:200), Sema3a (Abcam, ab23393, 1:100), Nrp1 (Abcam, ab81321, 1:100), and Myh4 (Invitrogen, 14-6503-82, 1:100). .. For immunohistochemistry of raw embedded frozen sections, the following antibodies were used: Twist2 (Abcam, ab66031, 1:200), Sema3a (Abcam, ab23393, 1:100), Nrp1 (Abcam, ab81321, 1:100), myosin IIa (Developmental Studies Hybridoma Bank, SC-71, 1:10), myosin IIb (Developmental Studies Hybridoma Bank, BF-F3, 1:10), Slow myosin (Sigma-Aldrich, NOQ7.5D, 1:250), and Fast myosin (Sigma-Aldrich, clone My32, 1:250). ..



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    anti-myosin heavy chain type iib  (Developmental Studies Hybridoma Bank)
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    myhc iib  (Developmental Studies Hybridoma Bank)
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    Immunofluorescence staining of the superficial region of the gastrocnemius muscle at (a) 90 and (b) 210 min post-feeding. Black indicates <t>MyHC</t> IIx–positive fibers, and green indicates <t>MyHC</t> <t>IIb–positive</t> fibers. The cross-sectional area of each fiber was calculated, and data are presented as the mean ± SEM. Statistical comparisons were performed using Student’s t -test (90 min: N = 3; 210 min: N = 4). *; p < 0.05, **; p < 0.01. Scale bar = 100 μm.
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    mhc type iib  (Developmental Studies Hybridoma Bank)
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    Immunofluorescence staining of the superficial region of the gastrocnemius muscle at (a) 90 and (b) 210 min post-feeding. Black indicates <t>MyHC</t> IIx–positive fibers, and green indicates <t>MyHC</t> <t>IIb–positive</t> fibers. The cross-sectional area of each fiber was calculated, and data are presented as the mean ± SEM. Statistical comparisons were performed using Student’s t -test (90 min: N = 3; 210 min: N = 4). *; p < 0.05, **; p < 0.01. Scale bar = 100 μm.
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    myhc type iib  (Developmental Studies Hybridoma Bank)
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    A: Representative immunofluorescence images of day 3 post-differentiation C2C12 myotubes that were treated during differentiation with various NSAIDs, including aspirin (ASA), indomethacin (INDO), ibuprofen (IBU), celecoxib, and SC-236 at the indicated concentrations. Cells were stained for myosin heavy chain <t>(MyHC,</t> green), myogenin (MyoG, red), and DAPI (nuclei, blue). B: Representative immunofluorescence images of day 3 post-differentiation C2C12 myotubes treated with doses of INDO ranging from 6.25 µM to 400 µM. C-E : Quantitative analysis of INDO-treated cells showing a dose-dependent decrease in DAPI + cell density ( C ), differentiation index (%) ( D ), and myotube area (%) ( E ). F: Representative images of ASA-treated myotubes ranging from 62.5 µM to 4 mM. H–J: Quantification of DAPI + cell density ( H ), differentiation index (%) ( I ), and myotube area (%) ( J ) in C2C12 cells receiving ASA treatment. K: Representative images comparing the effects of ASA (2 mM), INDO (200 µM), and the combination of INDO + ASA on C2C12 myotube morphology. L-N: Statistical comparison of cell density ( L ), differentiation index (%) ( M ), and myotube area (%) ( N ) across vehicle and treatment groups. Data are expressed as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Holm-Šídák post hoc tests. *, **, ***, and **** denotes p<0.05, p<0.01, p<0.001, and p<0.0001 between indicated groups, respectively. # denotes p<0.05 difference between ASA and vehicle groups.
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    myosin heavy chain type iib  (Developmental Studies Hybridoma Bank)
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    A: Representative immunofluorescence images of day 3 post-differentiation C2C12 myotubes that were treated during differentiation with various NSAIDs, including aspirin (ASA), indomethacin (INDO), ibuprofen (IBU), celecoxib, and SC-236 at the indicated concentrations. Cells were stained for myosin heavy chain <t>(MyHC,</t> green), myogenin (MyoG, red), and DAPI (nuclei, blue). B: Representative immunofluorescence images of day 3 post-differentiation C2C12 myotubes treated with doses of INDO ranging from 6.25 µM to 400 µM. C-E : Quantitative analysis of INDO-treated cells showing a dose-dependent decrease in DAPI + cell density ( C ), differentiation index (%) ( D ), and myotube area (%) ( E ). F: Representative images of ASA-treated myotubes ranging from 62.5 µM to 4 mM. H–J: Quantification of DAPI + cell density ( H ), differentiation index (%) ( I ), and myotube area (%) ( J ) in C2C12 cells receiving ASA treatment. K: Representative images comparing the effects of ASA (2 mM), INDO (200 µM), and the combination of INDO + ASA on C2C12 myotube morphology. L-N: Statistical comparison of cell density ( L ), differentiation index (%) ( M ), and myotube area (%) ( N ) across vehicle and treatment groups. Data are expressed as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Holm-Šídák post hoc tests. *, **, ***, and **** denotes p<0.05, p<0.01, p<0.001, and p<0.0001 between indicated groups, respectively. # denotes p<0.05 difference between ASA and vehicle groups.
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    anti myosin heavy chain type iib  (Developmental Studies Hybridoma Bank)
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    Developmental Studies Hybridoma Bank anti myosin heavy chain type iib
    A: Representative immunofluorescence images of day 3 post-differentiation C2C12 myotubes that were treated during differentiation with various NSAIDs, including aspirin (ASA), indomethacin (INDO), ibuprofen (IBU), celecoxib, and SC-236 at the indicated concentrations. Cells were stained for myosin heavy chain <t>(MyHC,</t> green), myogenin (MyoG, red), and DAPI (nuclei, blue). B: Representative immunofluorescence images of day 3 post-differentiation C2C12 myotubes treated with doses of INDO ranging from 6.25 µM to 400 µM. C-E : Quantitative analysis of INDO-treated cells showing a dose-dependent decrease in DAPI + cell density ( C ), differentiation index (%) ( D ), and myotube area (%) ( E ). F: Representative images of ASA-treated myotubes ranging from 62.5 µM to 4 mM. H–J: Quantification of DAPI + cell density ( H ), differentiation index (%) ( I ), and myotube area (%) ( J ) in C2C12 cells receiving ASA treatment. K: Representative images comparing the effects of ASA (2 mM), INDO (200 µM), and the combination of INDO + ASA on C2C12 myotube morphology. L-N: Statistical comparison of cell density ( L ), differentiation index (%) ( M ), and myotube area (%) ( N ) across vehicle and treatment groups. Data are expressed as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Holm-Šídák post hoc tests. *, **, ***, and **** denotes p<0.05, p<0.01, p<0.001, and p<0.0001 between indicated groups, respectively. # denotes p<0.05 difference between ASA and vehicle groups.
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    Image Search Results


    Immunofluorescence staining of the superficial region of the gastrocnemius muscle at (a) 90 and (b) 210 min post-feeding. Black indicates MyHC IIx–positive fibers, and green indicates MyHC IIb–positive fibers. The cross-sectional area of each fiber was calculated, and data are presented as the mean ± SEM. Statistical comparisons were performed using Student’s t -test (90 min: N = 3; 210 min: N = 4). *; p < 0.05, **; p < 0.01. Scale bar = 100 μm.

    Journal: PLOS One

    Article Title: Alaska pollock protein as a functional dietary source for promoting skeletal muscle hypertrophy and lipid metabolic remodeling

    doi: 10.1371/journal.pone.0348366

    Figure Lengend Snippet: Immunofluorescence staining of the superficial region of the gastrocnemius muscle at (a) 90 and (b) 210 min post-feeding. Black indicates MyHC IIx–positive fibers, and green indicates MyHC IIb–positive fibers. The cross-sectional area of each fiber was calculated, and data are presented as the mean ± SEM. Statistical comparisons were performed using Student’s t -test (90 min: N = 3; 210 min: N = 4). *; p < 0.05, **; p < 0.01. Scale bar = 100 μm.

    Article Snippet: Sections were fixed with acetone and incubated overnight at 4°C with primary antibodies against MyHC IIa (SC-71) and MyHC IIb (BF-F3), obtained from the Developmental Studies Hybridoma Bank (University of Iowa, Iowa City, IA, USA).

    Techniques: Immunofluorescence, Staining

    A: Representative immunofluorescence images of day 3 post-differentiation C2C12 myotubes that were treated during differentiation with various NSAIDs, including aspirin (ASA), indomethacin (INDO), ibuprofen (IBU), celecoxib, and SC-236 at the indicated concentrations. Cells were stained for myosin heavy chain (MyHC, green), myogenin (MyoG, red), and DAPI (nuclei, blue). B: Representative immunofluorescence images of day 3 post-differentiation C2C12 myotubes treated with doses of INDO ranging from 6.25 µM to 400 µM. C-E : Quantitative analysis of INDO-treated cells showing a dose-dependent decrease in DAPI + cell density ( C ), differentiation index (%) ( D ), and myotube area (%) ( E ). F: Representative images of ASA-treated myotubes ranging from 62.5 µM to 4 mM. H–J: Quantification of DAPI + cell density ( H ), differentiation index (%) ( I ), and myotube area (%) ( J ) in C2C12 cells receiving ASA treatment. K: Representative images comparing the effects of ASA (2 mM), INDO (200 µM), and the combination of INDO + ASA on C2C12 myotube morphology. L-N: Statistical comparison of cell density ( L ), differentiation index (%) ( M ), and myotube area (%) ( N ) across vehicle and treatment groups. Data are expressed as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Holm-Šídák post hoc tests. *, **, ***, and **** denotes p<0.05, p<0.01, p<0.001, and p<0.0001 between indicated groups, respectively. # denotes p<0.05 difference between ASA and vehicle groups.

    Journal: bioRxiv

    Article Title: Aspirin hastens resolution of skeletal muscle inflammation and promotes recovery of muscle strength following acute injury

    doi: 10.64898/2026.04.21.719989

    Figure Lengend Snippet: A: Representative immunofluorescence images of day 3 post-differentiation C2C12 myotubes that were treated during differentiation with various NSAIDs, including aspirin (ASA), indomethacin (INDO), ibuprofen (IBU), celecoxib, and SC-236 at the indicated concentrations. Cells were stained for myosin heavy chain (MyHC, green), myogenin (MyoG, red), and DAPI (nuclei, blue). B: Representative immunofluorescence images of day 3 post-differentiation C2C12 myotubes treated with doses of INDO ranging from 6.25 µM to 400 µM. C-E : Quantitative analysis of INDO-treated cells showing a dose-dependent decrease in DAPI + cell density ( C ), differentiation index (%) ( D ), and myotube area (%) ( E ). F: Representative images of ASA-treated myotubes ranging from 62.5 µM to 4 mM. H–J: Quantification of DAPI + cell density ( H ), differentiation index (%) ( I ), and myotube area (%) ( J ) in C2C12 cells receiving ASA treatment. K: Representative images comparing the effects of ASA (2 mM), INDO (200 µM), and the combination of INDO + ASA on C2C12 myotube morphology. L-N: Statistical comparison of cell density ( L ), differentiation index (%) ( M ), and myotube area (%) ( N ) across vehicle and treatment groups. Data are expressed as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Holm-Šídák post hoc tests. *, **, ***, and **** denotes p<0.05, p<0.01, p<0.001, and p<0.0001 between indicated groups, respectively. # denotes p<0.05 difference between ASA and vehicle groups.

    Article Snippet: Primary antibodies used include MyHC type I [Developmental Studies Hybridoma Bank (DSHB), BA-D5c, 1:100], MyHC type IIA (DSHB, SC-71c, 1:100), MyHC type IIB (DSHB, BF-F3c, 1:100), eMHC (DSHB, F1.652s, 1:20), Ly6G (GR1) (Bio-Rad, MCA2387, 1:50), CD68 (Bio-Rad, MCA1957, 1:200), CD206 (Bio-Rad, MCA2387, 1:50), and laminin (Abcam, ab7463, 1:200).

    Techniques: Immunofluorescence, Staining, Comparison